Coding
Part:BBa_K3619011:Design
Designed by: Isak Ingerholt Group: iGEM20_Lund (2020-09-13)
Gene scar, encoding the antimicrobial peptide Scarabaecin.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is codon optimized for expression in E.coli K12.
Source
The scar gene originates from Oryctes rhinoceros. DNA sequence was obtained from back translation of the peptide scarabaecin.
References
1. https://www.uniprot.org/uniprot/Q86SC0
2. https://www.sciencedirect.com/science/article/abs/pii/S0006291X03011628